Volume 4, Issue 3 (August 2025)
Health Science Monitor 2025, 4(3): 212-218 |
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Naseri F, Khademvatan S, Hazrati Tappeh K, Mahmoodzadeh S, Silivanova E A, Al-Muffti S A et al . Molecular Detection of Giardia Duodenalis Sub-Assemblages among Human Diarrheal Patients in Northwestern Iran. Health Science Monitor 2025; 4 (3) :212-218
URL: http://hsm.umsu.ac.ir/article-1-244-en.html
URL: http://hsm.umsu.ac.ir/article-1-244-en.html
Firoozeh Naseri 
, Shahram Khademvatan , Khosrow Hazrati Tappeh , Sama Mahmoodzadeh , Elena A. Silivanova , Shamal Abdullah Al-Muffti , Saber Gholizadeh *
, Shahram Khademvatan , Khosrow Hazrati Tappeh , Sama Mahmoodzadeh , Elena A. Silivanova , Shamal Abdullah Al-Muffti , Saber Gholizadeh *
Pathogens and Vectors Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
Abstract: (188 Views)
Background Giardia duodenalis is a prevalent intestinal protozoan parasite worldwide and consequently, in Iran. The diversity of Giardia genotypes infecting humans in Iran is poorly documented. In the current study, the frequency and diversity of G. duodenalis assemblages and sub-assemblages circulating among human patients with diarrheal symptoms were investigated.
Methods Fecal specimens were collected from 135 patients with diarrhea at health centers in Urmia, Iran, between 2014 and 2015. A total of 45 microscopically Giardia cyst-positive samples were used for DNA extraction, genotyping by PCR amplification, and sequence analysis of the Glutamate Dehydrogenase (gdh) and Triose-Phosphate Isomerase (tpi) loci.
Results Both gdh and tpi markers successfully genotyped in 76.3% (29/38) and 67.6% (25/37) isolates as assemblage A, respectively. All of the assemblage A strains belong to sub-assemblage AII with 100% similarity. Assemblage B isolates were categorized into sub-assemblages BIII (18.9%/7/12/37) and BIV (13.5%/5/12/37) with the tpi marker. The frequency of sub-assemblages BIII and BIV was 7.9% (3/9/29) and 15.8% (6/9/29) when using the gdh marker. Six isolates (13.95%) were genotyped as BIII by the tpi marker and as BIV by the gdh marker.
Conclusion It could be concluded that sub-assemblage AII is the prevalent sub-assemblage of G. duodenalis circulating among human diarrheal patients in Urmia, Iran. The sequence of gdh was more polymorphic than tpi in our analyzed samples.
Methods Fecal specimens were collected from 135 patients with diarrhea at health centers in Urmia, Iran, between 2014 and 2015. A total of 45 microscopically Giardia cyst-positive samples were used for DNA extraction, genotyping by PCR amplification, and sequence analysis of the Glutamate Dehydrogenase (gdh) and Triose-Phosphate Isomerase (tpi) loci.
Results Both gdh and tpi markers successfully genotyped in 76.3% (29/38) and 67.6% (25/37) isolates as assemblage A, respectively. All of the assemblage A strains belong to sub-assemblage AII with 100% similarity. Assemblage B isolates were categorized into sub-assemblages BIII (18.9%/7/12/37) and BIV (13.5%/5/12/37) with the tpi marker. The frequency of sub-assemblages BIII and BIV was 7.9% (3/9/29) and 15.8% (6/9/29) when using the gdh marker. Six isolates (13.95%) were genotyped as BIII by the tpi marker and as BIV by the gdh marker.
Conclusion It could be concluded that sub-assemblage AII is the prevalent sub-assemblage of G. duodenalis circulating among human diarrheal patients in Urmia, Iran. The sequence of gdh was more polymorphic than tpi in our analyzed samples.
Type of Study: Research |
Subject:
Special
Received: 2025/06/3 | Accepted: 2025/08/25 | Published: 2025/08/19
Received: 2025/06/3 | Accepted: 2025/08/25 | Published: 2025/08/19
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