Health Science Monitor
Health Science Monitor
Health Science Monitor
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http://hsm.umsu.ac.ir
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2022
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Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran
عمومى
General
پژوهشي
Research
<span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">:</span></b><i> </i><i><span style="font-size:9.0pt">Blastocystis</span></i><span style="font-size:9.0pt"> sp. is a common parasite of humans with a vast variety of nonhuman hosts and global distribution. Based on molecular methods, distinct subtypes are isolated from different hosts. In this study, we detected human-isolated <i>Blastocystis</i> subtypes in the northwestern of Iran. </span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Materials & Methods</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> A total of 600 stool samples referred to health centers in Urmia and Maragheh cities in the northwest of Iran was randomly collected and examined using direct wet mount, formalin-ether concentration, a modified version of the Ziehl–Neelsen staining technique for the detection of parasitological items<i> </i>and using barcoding method (18S rRNA gene) for the molecular survey. </span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Results</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> From 600 samples studied, 496 (82.7%) and 104 (17.3%) were male and female, respectively. Three subtypes, including ST<sub>1</sub>, ST<sub>2</sub> and ST<sub>3</sub>, were identified from collected samples, and the frequent subtypes were ST<sub>3</sub>, ST<sub>1,</sub> and ST<sub>2</sub>, respectively.</span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> While genetic similarity of <i>Blastocystis</i> isolated from human and animal did not show in this region, the zoonotic cycle of this parasite, probably by fecal contamination, exists in the study area. By identifying <i>Blastocystis</i> subtypes, it is possible to prevent the transmission and related complications. </span></span></span></span>
Blastocystis sp., 18S rRNA, Northwestern of Iran
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http://hsm.umsu.ac.ir/browse.php?a_code=A-10-111-1&slc_lang=en&sid=1
Mehrdad
Rostami
m.rostami9675@gmail.com
10031947532846001520
1111-1111-1111-1111
No
Department of Hematology and Blood Bank, Mashhad University of Medical Sciences, Mashhad, Iran
Arezoo
Bozorgomid
arezoobozorgomid@yahoo.com
10031947532846001521
10031947532846001521
No
Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Hadi
Mirahmadi
hmirahmadi59@gmail.com
10031947532846001522
10031947532846001522
No
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran AND Dept. of Parasitology, Mycology and Entomology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Saber
Raeghi
saberraeghi@gmail.com
10031947532846001523
10031947532846001523
Yes
Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran