en عمومى General پژوهشي Research Article <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b><span style="font-size:9.0pt">Methamphetamine is one of the most widely abused drugs worldwide. Its rapid and simple detection and determination in street drugs and biological samples are of great importance for forensic science applications.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Materials & Methods</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> In this work, a simple and sensitive chemiluminescence-based method was established for methamphetamine analysis.<b> </b>It is based on the enhancing effect of methamphetamine on Ce(IV)-Na₂SO₃ chemiluminescence (CL) reaction. A possible mechanism was discussed for the CL system. Optimization of chemical variables affecting the CL response of the system was performed by an experimental design approach using the central composite design. </span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Results</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> Under the optimum conditions, the enhanced CL intensity was proportional to the concentration of methamphetamine in the range of 0.005-5.0 &micro;g/mL, with a detection limit of 1.2 ng/mL. </span></span></span></span><br> <b><i><span style="font-size:9.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></i><span style="font-size:9.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">: </span></span></span></b><span style="font-size:9.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">The developed method applied to determine methamphetamine in street drugs and human plasma samples showed satisfactory results.</span></span></span> Chemiluminescence, Cerium (IV), Experimental design, Methamphetamine, Sodium sulfite 39 49 http://hsm.umsu.ac.ir/browse.php?a_code=A-10-84-1&slc_lang=en&sid=1 Tooba Hallaj t.hallaj@tabrizu.ac.ir 10031947532846001406 10031947532846001406 Yes Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran Mohammad Amjadi 10031947532846001407 10031947532846001407 No Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran Jamshid L. Manzoori 10031947532846001408 10031947532846001408 No Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran