en تخصصي Special پژوهشي Research Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:115%"><b><span new="" roman="" times="">Background</span></b><span new="" roman="" times=""> Giardia duodenalis is a prevalent intestinal protozoan parasite worldwide and consequently, in Iran. The diversity of Giardia genotypes infecting humans in Iran is poorly documented. In the current study, the frequency and diversity of G. duodenalis assemblages and sub-assemblages circulating among human patients with diarrheal symptoms were investigated.</span></span><br> <span style="line-height:115%"><b><span new="" roman="" times="">&nbsp;Methods</span></b><span new="" roman="" times=""> Fecal specimens were collected from 135 patients with diarrhea at health centers in Urmia, Iran, between 2014 and 2015. A total of 45 microscopically Giardia cyst-positive samples were used for DNA extraction, genotyping by PCR amplification, and sequence analysis of the Glutamate Dehydrogenase (gdh) and Triose-Phosphate Isomerase (tpi) loci.</span></span><br> <span style="line-height:115%"><b><span new="" roman="" times="">&nbsp;Results</span></b><span new="" roman="" times=""> Both gdh and tpi markers successfully genotyped in 76.3% (29/38) and 67.6% (25/37) isolates as assemblage A, respectively. All of the assemblage A strains belong to sub-assemblage AII with 100% similarity. Assemblage B isolates were categorized into sub-assemblages BIII (18.9%/7/12/37) and BIV (13.5%/5/12/37) with the tpi marker. The frequency of sub-assemblages BIII and BIV was 7.9% (3/9/29) and 15.8% (6/9/29) when using the gdh marker. Six isolates (13.95%) were genotyped as BIII by the tpi marker and as BIV by the gdh marker.</span></span><br> <span style="line-height:115%"><span new="" roman="" times="">&nbsp;<b>Conclusion</b> It could be concluded that sub-assemblage AII is the prevalent sub-assemblage of G. duodenalis circulating among human diarrheal patients in Urmia, Iran. The sequence of gdh was more polymorphic than tpi in our analyzed samples.</span></span><br> &nbsp;</span></span> Assemblages, Genotyping, Giardia duodenalis, Giardiasis, Iran, Urmia 212 218 http://hsm.umsu.ac.ir/browse.php?a_code=A-10-342-1&slc_lang=en&sid=1 Firoozeh Naseri f.naseri192@gmail.com 10031947532846004221 0009-0006-7007-3012 No Student Research Committee, University of Medical Sciences, Urmia, Iran Shahram Khademvatan shahramkhademvatan@gmail.com 10031947532846004222 10031947532846004222 No Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran Khosrow Hazrati Tappeh 10031947532846004223 10031947532846004223 No Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran Sama Mahmoodzadeh sama.mahmoodzadeh@gmail.com 10031947532846004224 0009-0001-4647-256X No Student Research Committee, University of Medical Sciences, Urmia, Iran Elena A. Silivanova 10031947532846004225 10031947532846004225 No All Russian Scientific Research Institute of Veterinary Entomology and Arachnology, Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS), Institutskaya st., 2, Tyumen 625041, Russian Federation, Russia Shamal Abdullah Al-Muffti 10031947532846004226 10031947532846004226 No Department of Biology, College of Science, University of Duhok, Kurdistan Region, Iraq Saber Gholizadeh sabergholizadehh@gmail.com 10031947532846004227 10031947532846004227 Yes Pathogens and Vectors Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran