en Health Protection including control of communicable and non-communicable diseases Health Protection including control of communicable and non-communicable diseases پژوهشي Research Article <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span style="font-size:9.0pt">The Ministry of Health and Treatment implemented vaccination as one of the primary strategies to control the <a name="_Hlk184712982">coronavirus disease 2019 (COVID-19)</a> pandemic. Given their critical role in treatment and disease control, healthcare workers were prioritized for vaccination. This study aims to assess the vaccination status and level of immunity against COVID-19 among healthcare workers at Shahid Motahari Hospital in Urmia, Iran, in 2021.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Materials & Methods</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> &nbsp;A cross-sectional study was conducted with 150 volunteer healthcare workers at Shahid Motahari Hospital. Venous blood samples were collected to measure <a name="_Hlk184713010">severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) </a>neutralizing antibodies using the ELISA method. Data analysis was performed using version 16 of SPSS software.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Results</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> The participants had a mean age of 37.5 years, with 47.3% being male. A significant 98% of the participants were vaccinated against COVID-19. The predominant vaccines administered were Sputnik V (72%), AstraZeneca (54%), and Sinopharm (27%). The mean level of SARS-CoV-2 neutralizing antibodies was significantly higher in vaccinated individuals compared to unvaccinated individuals (<i>p</i> = 0.001). There was no significant difference in antibody levels between male and female participants (<i>p</i> = 0.67). A positive correlation was observed between the number of vaccine doses received and the level of neutralizing antibodies (<i>R</i> = 0.207). </span></span></span></span><br> <b><i><span style="font-size:9.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></i><span style="font-size:9.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">: </span></span></span></b><span style="font-size:9.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Vaccination with the mentioned vaccines, whether administered singly or in combination, effectively increased serum antibody levels, providing sufficient protection against COVID-19 for healthcare workers. To maintain immunity levels among healthcare workers and protect against emerging strains of the virus, it is essential to administer booster doses.</span></span></span> COVID-19, Immunity level, Iran, Healthcare workers, Vaccination 7 14 http://hsm.umsu.ac.ir/browse.php?a_code=A-10-280-1&slc_lang=en&sid=1 Maryam Taavon maryam.taavon2021@gmail.com 10031947532846003419 10031947532846003419 No MSc student in Microbiology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran Vahid Tanhaei Marand dr_vahid9481@yahoo.com 10031947532846003420 10031947532846003420 Yes Assistant Professor, Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran Sadegh Feizollahzadeh sadeghimmune@yahoo.com 10031947532846003421 10031947532846003421 No Assistant Professor of Immunology, Department of Immunology and Genetics, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran